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xlink:href="#svg-arrow-right"></use></svg> </a><a href="guide.html" class="nav-list-link">Guide</a><ul class="nav-list"><li class="nav-list-item "><a href="guide/1-start.html" class="nav-list-link">1 - Getting Started</a></li><li class="nav-list-item "><a href="guide/2-supplies.html" class="nav-list-link">2 - What You'll Need</a></li><li class="nav-list-item "><a href="guide/3-work-area.html" class="nav-list-link">3 - Creating a Work Area</a></li><li class="nav-list-item "><a href="guide/4-sterilize.html" class="nav-list-link">4 - Wash and Sterilize Tools and Vials</a></li><li class="nav-list-item "><a href="guide/5-mix.html" class="nav-list-link">5 - Mix Solution</a></li><li class="nav-list-item "><a href="guide/6-filter.html" class="nav-list-link">6 - Filter Solution</a></li><li class="nav-list-item "><a href="guide/7-bubble-point.html" class="nav-list-link">7 - Bubble Point Test</a></li><li class="nav-list-item "><a href="guide/8-dispense.html" class="nav-list-link">8 - Dispense Solution</a></li><li class="nav-list-item "><a href="guide/9-final-steps.html" class="nav-list-link">9 - Final Steps</a></li><li class="nav-list-item "><a href="guide/10-selling.html" class="nav-list-link">10 - Running a Business</a></li></ul></li><li class="nav-list-item"><a href="summary.html#" class="nav-list-expander" aria-label="toggle links in Topics category"> <svg viewBox="0 0 24 24"><use xlink:href="#svg-arrow-right"></use></svg> </a><a href="hrtcat/topics.html" class="nav-list-link">Topics</a><ul class="nav-list"><li class="nav-list-item "><a href="hrtcat/topics/make_agar.html" class="nav-list-link">Agar Plates</a></li><li class="nav-list-item "><a href="hrtcat/topics/alcohol_technique.html" class="nav-list-link">Alcohol Sanitization Technique</a></li><li class="nav-list-item "><a href="hrtcat/topics/biological_indicators.html" class="nav-list-link">Biological Indicators</a></li><li class="nav-list-item "><a href="hrtcat/topics/bulk_filtration.html" class="nav-list-link">Bulk Filtration</a></li><li class="nav-list-item "><a href="hrtcat/topics/choosing_oil.html" class="nav-list-link">Carrier Oils</a></li><li class="nav-list-item "><a href="hrtcat/topics/esters_compared.html" class="nav-list-link">Esters Compared</a></li><li class="nav-list-item "><a href="hrtcat/topics/fingertip_testing.html" class="nav-list-link">Fingertip Testing</a></li><li class="nav-list-item "><a href="hrtcat/topics/hormone_testing.html" class="nav-list-link">Hormone Testing</a></li><li class="nav-list-item "><a href="topics/incubation.html" class="nav-list-link">Incubation</a></li><li class="nav-list-item "><a href="hrtcat/topics/instant_pot.html" class="nav-list-link">Instant Pot Sterilization</a></li><li class="nav-list-item "><a href="hrtcat/topics/diy_laminar.html" class="nav-list-link">Laminar Flow Hood</a></li><li class="nav-list-item "><a href="hrtcat/topics/recipes.html" class="nav-list-link">Recipes</a></li><li class="nav-list-item "><a href="hrtcat/topics/sanitize_sterilize_depyrogenate.html" class="nav-list-link">Sanitize vs Sterilize vs Depyrogenate</a></li><li class="nav-list-item "><a href="hrtcat/topics/sterilization_temps.html" class="nav-list-link">Sterilization Temperatures</a></li><li class="nav-list-item "><a href="hrtcat/topics/still_air.html" class="nav-list-link">Still Air Boxes</a></li><li class="nav-list-item "><a href="hrtcat/topics/choosing_filter.html" class="nav-list-link">Syringe Filters</a></li><li class="nav-list-item "><a href="hrtcat/topics/vial_size_concentration.html" class="nav-list-link">Vial Sizes and Concentrations</a></li></ul></li><li class="nav-list-item"><a href="summary.html#" class="nav-list-expander" aria-label="toggle links in Experiments category"> <svg viewBox="0 0 24 24"><use xlink:href="#svg-arrow-right"></use></svg> </a><a href="experiments.html" class="nav-list-link">Experiments</a><ul class="nav-list"><li class="nav-list-item "><a href="experiments/benzyl_benzoate_concentration.html" class="nav-list-link">Benzyl Benzoate Concentration</a></li><li class="nav-list-item "><a href="experiments/sterilizing_vials.html" class="nav-list-link">Sterilizing Vials</a></li></ul></li><li class="nav-list-item active"><a href="summary.html#" class="nav-list-expander" aria-label="toggle links in Other category"> <svg viewBox="0 0 24 24"><use xlink:href="#svg-arrow-right"></use></svg> </a><a href="other.html" class="nav-list-link">Other</a><ul class="nav-list"><li class="nav-list-item "><a href="journal.html" class="nav-list-link">Journal</a></li><li class="nav-list-item "><a href="calculator.html" class="nav-list-link">Recipe Calculator</a></li><li class="nav-list-item active"><a href="summary.html" class="nav-list-link active">Guide Summary</a></li><li class="nav-list-item "><a href="anonymity.html" class="nav-list-link">Maintaining Anonymity</a></li><li class="nav-list-item "><a href="lena.html" class="nav-list-link">A Critique of Lena's Method</a></li><li class="nav-list-item "><a href="where-to-order.html" class="nav-list-link">Where to Order HRT</a></li><li class="nav-list-item "><a href="producers.html" class="nav-list-link">Known HRT Producers</a></li><li class="nav-list-item "><a href="properly-sterilized.html" class="nav-list-link">Were Your DIY Vials Properly Sterilized?</a></li><li class="nav-list-item "><a href="start-hrt.html" class="nav-list-link">Starting HRT</a></li><li class="nav-list-item "><a href="lidocaine.html" class="nav-list-link">DIY Topical Lidocaine Cream</a></li><li class="nav-list-item "><a href="network.html" class="nav-list-link">HRT Cat Network</a></li></ul></li><li class="nav-list-item"><a href="media.html" class="nav-list-link">Shareable Media</a></li><li class="nav-list-item"><a href="contribute.html" class="nav-list-link">Contribute</a></li><li class="nav-list-item"><a href="contact.html" class="nav-list-link">Contact</a></li><li class="nav-list-item"><a href="donate.html" class="nav-list-link">Donate</a></li><li class="nav-list-item"><a href="resources.html" class="nav-list-link">Additional Resources</a></li><li class="nav-list-item"><a href="changelog.html" class="nav-list-link">Changelog</a></li></ul> </nav> <footer class="site-footer"> This site uses <a href="https://github.com/just-the-docs/just-the-docs">Just the Docs</a>, a documentation theme for Jekyll. </footer> </div> <div class="main" id="top"> <div id="main-header" class="main-header"> <div class="search"> <div class="search-input-wrap"> <input type="text" id="search-input" class="search-input" tabindex="0" placeholder="Search HRT Cat" aria-label="Search HRT Cat" autocomplete="off"> <label for="search-input" class="search-label"><svg viewBox="0 0 24 24" class="search-icon"><use xlink:href="#svg-search"></use></svg></label> </div> <div id="search-results" class="search-results"></div> </div> </div> <div id="main-content-wrap" class="main-content-wrap"> <nav aria-label="Breadcrumb" class="breadcrumb-nav"> <ol class="breadcrumb-nav-list"> <li class="breadcrumb-nav-list-item"><a href="other.html">Other</a></li> <li class="breadcrumb-nav-list-item"><span>Guide Summary</span></li> </ol> </nav> <div id="main-content" class="main-content" role="main"> <h1 id="guide-summary"> <a href="summary.html#guide-summary" class="anchor-heading" aria-labelledby="guide-summary"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> Guide Summary </h1> <p>This is an example of the exact procedure we perform when brewing in order to help us have a quick reference sheet. It contains no explanations and just has day-of procedures.</p> <ul> <li>Clear work area</li> <li>Sweep and mop</li> <li>Hang plastic sheets</li> <li>Install and turn on HEPA air purifier</li> <li>Sanitize work surface</li> <li>Clean floor once more</li> <li>Begin wearing shoe covers and garbs in clean room</li> <li>Preheat oven to 200°C</li> <li>Wash, rinse, and wrap items for depyrogenation</li> <li>Put in oven once pre-heated, set timer for 2 hours</li> <li>Prepare autoclave with distilled water</li> <li>Wash, rinse, and wrap items for sterilization</li> <li>Autoclave for 1 hour.</li> <li>Wipe down everything else with IPA</li> <li>Setup laminar flow hood, leave on</li> <li>Remove items from oven and autoclave, stage in clean room</li> <li>Perform fingertip testing</li> <li>Inside flow hood, mix in beaker: <ul> <li>____mL MCT oil</li> <li>____mL Benzyl Alcohol</li> <li>____mL Benzyl Benzoate</li> <li>____mg Estradiol Enanthate</li> </ul> </li> <li>Filter ____mL aside for sterility testing<sup>+</sup></li> <li>Filter rest into vials, place stoppers immediately. Repeat.</li> <li>Use clean filter to do a second pass filtration into vials, cap<sup>+</sup>.</li> <li>Set 2nd pass filter aside for sterility testing<sup>+</sup>.</li> <li>Cap.</li> <li>Bubble point test <ul> <li>Listed bubble point: ____psi</li> <li>Today’s air pressure: ____psi</li> <li>Starting air volume: ____mL</li> <li>Actual bubble point volume: ____mL</li> <li>Actual bubble point (<a href="https://www.omnicalculator.com/physics/boyles-law">calculator</a>): ____psi</li> </ul> </li> <li>Place 2nd pass filter on agar for testing<sup>+</sup>.</li> <li>Make a record</li> <li>Label vials</li> <li>Incubate fingertip tests</li> </ul> </div> </div> <div class="search-overlay"></div> </div> </body> </html>
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