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xlink:href="#svg-arrow-right"></use></svg> </a><a href="../guide.html" class="nav-list-link">Guide</a><ul class="nav-list"><li class="nav-list-item "><a href="1-start.html" class="nav-list-link">1 - Getting Started</a></li><li class="nav-list-item "><a href="2-supplies.html" class="nav-list-link">2 - What You'll Need</a></li><li class="nav-list-item "><a href="3-work-area.html" class="nav-list-link">3 - Creating a Work Area</a></li><li class="nav-list-item "><a href="4-sterilize.html" class="nav-list-link">4 - Wash and Sterilize Tools and Vials</a></li><li class="nav-list-item active"><a href="5-mix.html" class="nav-list-link active">5 - Mix Solution</a></li><li class="nav-list-item "><a href="6-filter.html" class="nav-list-link">6 - Filter Solution</a></li><li class="nav-list-item "><a href="7-bubble-point.html" class="nav-list-link">7 - Bubble Point Test</a></li><li class="nav-list-item "><a href="8-dispense.html" class="nav-list-link">8 - Dispense Solution</a></li><li 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class="nav-list-item "><a href="hrtcat/topics/choosing_oil.html" class="nav-list-link">Carrier Oils</a></li><li class="nav-list-item "><a href="hrtcat/topics/esters_compared.html" class="nav-list-link">Esters Compared</a></li><li class="nav-list-item "><a href="hrtcat/topics/fingertip_testing.html" class="nav-list-link">Fingertip Testing</a></li><li class="nav-list-item "><a href="hrtcat/topics/hormone_testing.html" class="nav-list-link">Hormone Testing</a></li><li class="nav-list-item "><a href="../topics/incubation.html" class="nav-list-link">Incubation</a></li><li class="nav-list-item "><a href="hrtcat/topics/instant_pot.html" class="nav-list-link">Instant Pot Sterilization</a></li><li class="nav-list-item "><a href="hrtcat/topics/diy_laminar.html" class="nav-list-link">Laminar Flow Hood</a></li><li class="nav-list-item "><a href="hrtcat/topics/recipes.html" class="nav-list-link">Recipes</a></li><li class="nav-list-item "><a href="hrtcat/topics/sanitize_sterilize_depyrogenate.html" class="nav-list-link">Sanitize vs Sterilize vs Depyrogenate</a></li><li class="nav-list-item "><a href="hrtcat/topics/sterilization_temps.html" class="nav-list-link">Sterilization Temperatures</a></li><li class="nav-list-item "><a href="hrtcat/topics/still_air.html" class="nav-list-link">Still Air Boxes</a></li><li class="nav-list-item "><a href="hrtcat/topics/choosing_filter.html" class="nav-list-link">Syringe Filters</a></li><li class="nav-list-item "><a href="hrtcat/topics/vial_size_concentration.html" class="nav-list-link">Vial Sizes and Concentrations</a></li></ul></li><li class="nav-list-item"><a href="5-mix.html#" class="nav-list-expander" aria-label="toggle links in Experiments category"> <svg viewBox="0 0 24 24"><use xlink:href="#svg-arrow-right"></use></svg> </a><a href="../experiments.html" class="nav-list-link">Experiments</a><ul class="nav-list"><li class="nav-list-item "><a href="../experiments/benzyl_benzoate_concentration.html" class="nav-list-link">Benzyl Benzoate Concentration</a></li><li class="nav-list-item "><a href="../experiments/sterilizing_vials.html" class="nav-list-link">Sterilizing Vials</a></li></ul></li><li class="nav-list-item"><a href="5-mix.html#" class="nav-list-expander" aria-label="toggle links in Other category"> <svg viewBox="0 0 24 24"><use xlink:href="#svg-arrow-right"></use></svg> </a><a href="../other.html" class="nav-list-link">Other</a><ul class="nav-list"><li class="nav-list-item "><a href="../journal.html" class="nav-list-link">Journal</a></li><li class="nav-list-item "><a href="../calculator.html" class="nav-list-link">Recipe Calculator</a></li><li class="nav-list-item "><a href="../summary.html" class="nav-list-link">Guide Summary</a></li><li class="nav-list-item "><a href="../anonymity.html" class="nav-list-link">Maintaining Anonymity</a></li><li class="nav-list-item "><a href="../lena.html" class="nav-list-link">A Critique of Lena's Method</a></li><li class="nav-list-item "><a href="../where-to-order.html" class="nav-list-link">Where to Order HRT</a></li><li class="nav-list-item "><a href="../producers.html" class="nav-list-link">Known HRT Producers</a></li><li class="nav-list-item "><a href="../properly-sterilized.html" class="nav-list-link">Were Your DIY Vials Properly Sterilized?</a></li><li class="nav-list-item "><a href="../start-hrt.html" class="nav-list-link">Starting HRT</a></li><li class="nav-list-item "><a href="../lidocaine.html" class="nav-list-link">DIY Topical Lidocaine Cream</a></li><li class="nav-list-item "><a href="../network.html" class="nav-list-link">HRT Cat Network</a></li></ul></li><li class="nav-list-item"><a href="../media.html" class="nav-list-link">Shareable Media</a></li><li class="nav-list-item"><a href="../contribute.html" class="nav-list-link">Contribute</a></li><li class="nav-list-item"><a href="../contact.html" class="nav-list-link">Contact</a></li><li class="nav-list-item"><a href="../donate.html" class="nav-list-link">Donate</a></li><li class="nav-list-item"><a href="../resources.html" class="nav-list-link">Additional Resources</a></li><li class="nav-list-item"><a href="../changelog.html" class="nav-list-link">Changelog</a></li></ul> </nav> <footer class="site-footer"> This site uses <a href="https://github.com/just-the-docs/just-the-docs">Just the Docs</a>, a documentation theme for Jekyll. </footer> </div> <div class="main" id="top"> <div id="main-header" class="main-header"> <div class="search"> <div class="search-input-wrap"> <input type="text" id="search-input" class="search-input" tabindex="0" placeholder="Search HRT Cat" aria-label="Search HRT Cat" autocomplete="off"> <label for="search-input" class="search-label"><svg viewBox="0 0 24 24" class="search-icon"><use xlink:href="#svg-search"></use></svg></label> </div> <div id="search-results" class="search-results"></div> </div> </div> <div id="main-content-wrap" class="main-content-wrap"> <nav aria-label="Breadcrumb" class="breadcrumb-nav"> <ol class="breadcrumb-nav-list"> <li class="breadcrumb-nav-list-item"><a href="../guide.html">Guide</a></li> <li class="breadcrumb-nav-list-item"><span>5 - Mix Solution</span></li> </ol> </nav> <div id="main-content" class="main-content" role="main"> <h1 id="mix-solution"> <a href="5-mix.html#mix-solution" class="anchor-heading" aria-labelledby="mix-solution"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> Mix Solution </h1> <p><em>Updated July 21, 2023</em></p> <p class="info">We do not use a magnetic stirrer but that is an option instead of using a glass rod. Would be useful if making large batches.</p> <h2 id="recipe"> <a href="5-mix.html#recipe" class="anchor-heading" aria-labelledby="recipe"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> Recipe </h2> <p>Choose a recipe to brew, there are many options listed <a href="../topics/recipes.html">here</a>.</p> <p>You can also try out our new <a href="../calculator.html">recipe calculator here</a>! This calculator is in beta, so please use it with caution.</p> <h2 id="mixing"> <a href="5-mix.html#mixing" class="anchor-heading" aria-labelledby="mixing"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> Mixing </h2> <p>Doing this all inside your still air box or laminar flow hood. Using proper sanitization techniques.</p> <p>Measure out all the ingredients into a depyrogenated beaker or media bottle. They do not need to be added in any specific order. Mix them up with a glass stirring rod until the hormone powder is fully dissolved. If you are having trouble getting the powder into solution it is possible that you did not use enough benzyl benzoate.</p> <h2 id="if-using-heat"> <a href="5-mix.html#if-using-heat" class="anchor-heading" aria-labelledby="if-using-heat"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> If using heat </h2> <p>Heat is not required to get your hormone powder to dissolve, especially when you’re using benzyl benzoate in your mixture. However, the use of heat may accelerate the process of dissolving it, especially when making very high concentrations or leaving out BB. Without BB you risk it falling out of solution later with temperature fluctuations.</p> <p>When we assemble our cleanroom we fill a large thermos with boiling water, so we don’t have to boil water in the clean room. We then take a large bowl, pour some boiling water into it, and then submerge the beaker into the water while stirring. It massively speeds up the dissolving process.</p><hr /> <h2 id="navigation"> <a href="5-mix.html#navigation" class="anchor-heading" aria-labelledby="navigation"><svg viewBox="0 0 16 16" aria-hidden="true"><use xlink:href="#svg-link"></use></svg></a> Navigation </h2> <p><a href="4-sterilize.html" class="btn btn-purple">← 4. Wash and Sterilize Tools and Vials</a> <a href="6-filter.html" class="btn btn-purple">6. Filter Solution →</a></p> </div> </div> <div class="search-overlay"></div> </div> </body> </html>
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